Educational Program

The GRK1459 established a monthly seminar series which was open to the public and where world-leading scientists in the field of protein trafficking and protein-protein interactions were invited as guest speakers. Participation was mandatory for GRK1459 graduate students. To be better prepared to the public talk, the guest speakers gave an additional special seminar for the RTG doctoral researchers only, followed by a common dinner/lunch to allow personal contacts and discussion of their own research data.

Guest speakers of GRK1459 seminars from 10/2008 to 04/2017

2008/2009 (Seminar table as PDF)
Reinhard Jahn – MPI f. biophysikalische Chemie, Göttingen; Felix Wieland – Univ. Heidelberg; Volker Hauke – FMP, Berlin; Jean Gruenberg – Univ. Genf; Graham Warren – Max F. Perutz Laboratories, Wien; Tom Rapoport – Harvard Univ., Boston; Albert Haas – Univ. Bonn; Nils Brose – MPI f. Experimentelle Medizin, Göttingen

2009/2010 (Seminar table as PDF)
Juan Bonifacino – NIH, Bethesda; Hubert Hilbi – Univ. Zürich; Bernhard Hoflack – TU Dresden; Kai Simons – MPI-CBG, Dresden; Paul Luzio – Univ. of Cambridge; Philippe Chavrier – Inst. Curie, Paris; Harald Stenmark – Oslo Univ. Hospital

2010/2011 (Seminar table as PDF)
Norma Andrews – Univ. of Maryland; Suzanne Pfeffer – Stanford Univ.; Derek Toomre – Yale Univ., New Haven; Sandra Schmid – Univ. of California, San Diego; Nikolaus Pfanner – Univ. Freiburg; Ari Helenius – ETH Zürich; Michael Marks – Univ. of Pennsylvania; Wim Annaert – VIB, Leuven; Felix Wieland – Univ. Heidelberg; James Rothman – Yale Univ., New Haven

2011/2012 (Seminar table as PDF)
Oliver Daumke – MDC, Berlin; Thijn Brummelkamp – Whitehead Inst., Cambridge; Sergio Grinstein – Toronto Univ.; Anne Spang – Univ. Basel; Graca Raposo – Inst. Curie, Paris; Margaret Robinson – Univ. of Cambridge; Oddmund Bakke – Oslo Univ.; Ed Hurt – Univ. Heidelberg

2012/2013 (Seminar table as PDF)
Nicolas Demaurex– Univ. Genf; Ana-Maria Lennon-Duménil – Inst. Curie, Paris; Mikael Simons – MPI f. Experimentelle Medizin, Göttingen; Michael Forgac – Tufts Univ. Boston; Jodi Nunnari – Univ. of California, Davis; Peter Walter – Univ. of California, San Francisco; Andrea Ballabio – TIGEM, Pozzuoli; Vivek Malhotra – Centre for Genomic Regulation, Barcelona

2013/2014 (Seminar table as PDF)
David Holden – Imperial College London; Thomas Willnow – MDC, Berlin; Antonella De Matteis – TIGEM, Pozzuoli; Andreas Mayer – Univ. Lausanne; Graham Warren– Max F. Perutz Laboratories, Wien; Gia Voeltz – Univ. of Colorado, Boulder; Juan Bonifacino – NIH, Bethesda; Randy Schekman – Univ. of California, Berkeley

2014/2015 (Seminar table as PDF)
Arnaud Echard – Inst. Pasteur, Paris; Jean Gruenberg – Univ. Genf; Etienne Pays – Univ. libre de Bruxelles; Marino Zerial – MPI-CBG, Dresden; Prisca Liberali – Univ. Zürich; Lukas Kapitein – Utrecht Univ.; Tom Rapoport – Harvard Univ., Boston; Tamas Balla – NIH, Bethesda

2015/2016 (Seminar table as PDF)
David Rubinsztein – Univ. of Cambridge; Thomas Langer – Univ. Köln; Elina Ikonen – Univ. of Helsinki; Christian Hübner – Univ. Jena; Arwen Pearson – CUI Hamburg; Bruno Goud – Inst. Curie, Paris; Romeo Ricci – IGBMC, Strasbourg; Soman Abraham – Duke Univ., Durham

2016/2017 (Seminar table as PDF)
David Harris – Boston Univ.; Marko Kaksonen – Univ. Genf; Emmanuel Boucrot – Univ. College London; Jost Enninga – Inst. Pasteur, Paris; Xianping Dong – Dalhousie Univ., Halifax; Tamotsu Yoshimori – Osaka Univ.

The lecture series “Molecular Cell Biology of Subcellular Compartments“ was held at the beginning of each new generation of graduate students to introduce the basics of the GRK1459 research programme. Lectures were held by the PIs of the research training group.

Lectures held for 3rd generation of graduate students:
Nucleus (Hans-Jürgen Kreienkamp)
Endoplasmic reticulum (Sandra Pohl)
Golgi apparatus (Stephan Storch)
Endosomes (Judith Blanz)
Neuronal synapses (Matthias Kneussel)
Phagosomes/microbial compartments (Martin Aepfelbacher)
Cytoskeleton and intracellular transport (Stefan Linder)
Autophagy and aggregates (Markus Glatzel)
Peroxisomes (Jörg Heeren)
Lysosomes (Paul Saftig)
Subcellular compartments in parasites (Tobais Spielmann)
Plasmamembranes  (Michael Schwake)

The practical courses were organized and conducted by the participating research groups of the RTG 1459 and conveyed molecular-biological, microbiological, biochemical and biophysical methods that were routinely applied in the hosting research group. Participation in at least three courses was mandatory for the graduate students and usually the graduate students were also involved in conducting the course of his/her own research group. The pratical courses run for 3 days and were carried out in small groups of maximum of 4 students, giving the student the opportunity to not only learn new techniques but also to meet other researchers RTG1459’s research groups and get insight into other groups’ lab life.

Practical courses in 2016 (3rd generation of graduate students)
Pohl group, UKE: Synthesis and transport of lysosomal enzymes: Pulse-chase [35S]-methione labeling of MEFs and immunoprecipitation of cathepsin Z
Spielmann group, BNITM: 4D confocal microscopy of malaria blood stage parasites to visualise uptake of hemoglobin
Glatzel group, UKE: Methods of antigen retrieval of paraffin-embedded histological specimen
Aepfelbacher group, UKE: Live imaging of phagocytosis by human umbilical vein endothelial cells with invasin-coated microbeads
Linder group , UKE: Borrelia phagocytosis by human macrophages as a model for confocal imaging, ratiometric analysis and vesicle trafficking
Storch group, UKE: Analysis of endocytic pathways by density gradient centrifugation of cell homogenates
Kreienkamp group, UKE: Preparation and cultivation of neuronal cells
Heeren group, UKE: Receptor-mediated endocytosis and intracellular processing of lipoproteins
Kneussel group, ZMNH: Live cell imaging of intracellular transport processes using epifluorescent and spinning disk confocal microscopy
Saftig group, CAU Kiel: Ratiometric imaging to determine endosomal/lysosomal pH

Each graduate student of the RTG 1459 was given the opportunity to spend up to three month in a collaborating lab abroad to learn new techniques, benefit from other lab’s expertises to bring forward the own research project and strengthen the personal network with international colleagues.

Research stays abroad of graduate students (2010 – 2016)
* resulting joint publication of graduate student and hosting PI abroad

Kristin Albers – University of Cambridge, UK
Alexandra Blancke Soares – University of Utrecht, The Netherlands
Laura Brandenstein – University of Cambridge, UK
Raffaella De Pace – University of California, San Francisco, USA
Gioriga Di Lorenzo – Institut Curie, Paris, France
Clemens Falker* – Institute of Neurosience, Milano, Italy
Alexander Fischer* – Harvard T.H. Chan School of Public Health, Boston, USA
Sven Flemming – University of Cambridge, UK
Mine Franke – University of California, San Diego, USA
Saskia Heybrock – University of Helsinki, Finland
Malte Klüssendorf – McGill University, Montreal, Canada
Judith Koliwer* – University of California, San Fancisco, USA
Lucia Krott* – University of California, San Diego, USA
Dorothee Lasrich* – University of Sydney, Australia
María José Valencia López – University of Texas Southwestern Medical Center, USA
Sandra Markmann – University of California, San Diego, USA
Xenia Naj* – The Hospital for Sick Children, Toronto, Canada
Miriam Oelbe – UMC Utrecht, Netherlands
Liane Rauch* – Yale University, USA
Anja Röder* – Yale University, USA
Liliana Rojas-Charry – University of Ferrara, Italy
Ulrike Ruch* – Burnet Institute, Melbourne, Australia
Pieter Steenhuis – NIH, USA
Dana Thurm – Yale University, USA
Vanessa van Rahden* – Stanford University, USA
Jörn Michael Völker* – University of Lausanne, Switzerland