Regulated sorting of plasma membrane receptors through the Golgi-associated protein PIST
Prof. Dr. Hans-Jürgen Kreienkamp (2008 – 2017)
University Medical Center Hamburg-Eppendorf, Institute of Human Genetics
PIST/GOPC is a Golgi protein which has been implicated in in the sorting and intracellular transport of various receptor proteins. PIST is tethered to the trans-Golgi network through interactions with the GTPase Rab6 and syntaxin-6; in neurons it is present in somatic TGN and in Golgi outposts. Live cell imaging and biochemical assays indicate that PIST is stationary at these sites and does not participate in vesicular transport to the plasma membrane. We will analyze the relevance of PIST for targeting of receptors to postsynaptic sites in neurons. In particular, we will determine how the distribution of PIST-associated membrane proteins neuroligin-2 and stargazin is altered in the absence of PIST, and how PIST contributes to the regulation of membrane protein availibility at the cell surface. These experiments will contribute to our understanding of synapse formation and synaptic plasticity.